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TIANGEN--new product introductio

Date:2012-11-02   Browsing times:6609

I’m very glad to inform you that with the great effect of TIANGEN R&D and Manufacture Department, our new product ----FastQuant RT Kit; SuperReal PreMix Plus (SYBR Green).

FastQuant RT Kit is designed for the first strand cDNA synthesis from tiny amount of total RNA or mRNA for two-step RT-PCR which could clean up the genomic DNA effectively by gDNase under 42℃ within 3min and protect the total RNA from interference of genomic DNA. FastQuant RT Enzyme (reverse transcriptase contained in this kit) has a high affinity for RNA, which enables any template including the high GC percentage one and the template with complex second level structure be reverse transcribed efficiently and sensitively of, leading to high yields of cDNA. FastQuant RT Kit ensures that you get the cDNA-frist strand within 15min.

gDNA clean up expriment

gDNA clean up experiment shows that, the kit could clean up the genomic DNA totally within 3min. Production could be used to real-time qPCR directly without any process of RNA purification.

Finish the whole process within only 21min

Strong reading ability of super complex template

Fast quant kit has higher reverse transcription ability no matter with templates contain high GC percentage or complex second level structure.

SuperReal PreMix Plus (SYBR Green) is specially designed to perform Real-time PCR in SYBR Green I fluorescent-based detection assays. The Real-Time PCR reaction buffer, a 2× pre-mixed solution included in this kit, balanced the K+/NH4+ percentage and added a special H-bond factor which can regulate the H-bond function throughout the whole reaction process, enhancing the primer-template specificity and guaranteeing your experiment a better repetitiveness!

SuperReal PreMix Plus provides you a convenient format to highly sensitive and specific qPCR amplification.

SuperReal PreMix Plus (SYBR Green) makes your experiment better, faster, stronger.

Powerful role of H-bond factor

Result shows that reaction system containing H-bond could reduce the non-specific amplification effectively. Unique double hot-start enzymes regulate system SuperReal PreMix Plus adopts a unique dual hot-start enzymes system (chemically modified HotStar Taq DNA polymerase and antibody modified Anti Taq DNA Polyerase), which, plus the pre-optimized buffer solution, provides a convenient format for highly sensitive and specific qPCR amplification.

It makes the system the high amplification efficiency, high amplification specificity and broad trusted range.

Broad applications for the equipment of different brands.

SuperReal PreMix Plus could work different brand PCR instruments including ABI (PRISM 7000/7300/7700/7900HT/step one/7500/7500 fast), Stratagen (Mx3000p/Mx3005p/M4000) Roche, Bio-rad and Eppendorf, giving a stable result for your experiment.

Pro-stained Protein Marker (Multicolor Broad Range) is a kind of ready-to –use protein marker with 12 pre-stained proteins covering a wide range molecular weights from 11 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE. The Pro-stained Protein Marker (Multicolor Broad Range) is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

 

Basic information:

 Cat.No.  Product  Size
 KR106-01  FastQuant RT Kit(With gDNase)  25preps
 KR106-02  FastQuant RT Kit(With gDNase)  100preps
 FP205-01  SuperReal PreMix Plus(SYBR Green)  20ul×125preps/50ul×50preps
 FP205-02  SuperReal PreMix Plus(SYBR Green)  20ul×500preps/50ul×200 preps
 FP205-03  SuperReal PreMix Plus(SYBR Green)  20ul×5000preps/50ul×2000 preps
 MP206  Pro-stained Protein Marker  100 ul

 

 

 

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